Simple Brachial Venipuncture Technique in Large Softshell Turtles (Nilssonia spp.)

Abstract

Abstract The collection of blood from large soft-shell turtles, such as the threatened black softshell turtle (Nilssonia nigricans), Indian softshell turtle (Nilssonia gangetica) and the peacock softshell turtle (Nilssonia hurum), has been a challenge for researchers due to their aggressive nature and thick skin folds. In this pilot study, a technique was developed for blood collection from these turtles, which involves physical restraint and venipuncture of the brachial venous plexus. The method proved to be effective, efficient, and requires no anesthesia, making it suitable for field studies or on-site collection. Blood samples were successfully collected from 30 turtles captured, and basic hematology and biochemical examinations were performed. Based on our study, this method can be used for both large and small-sized animals and provides a reliable technique for routine health assessments in these species.