In vivo topical delivery of ethosomal formulation composed of Orthosiphon stamineus extract complexed with sophorolipid against melanoma

Abstract

Event Abstract Back to Event In vivo topical delivery of ethosomal formulation composed of Orthosiphon stamineus extract complexed with sophorolipid against melanoma Mansoureh Nazari Vishkaei1, Mohamed Khadeer Ahamed Basheer2 and Amin Malik Shah Abdul Majid3* 1 School of Pharmaceutical Sciences, University of Science Malaysia, Malaysia 2 Emanbiodiscoveries.sdn.bhd., Malaysia 3 University of Science, Malaysia, Malaysia Background Melanoma is one of the most aggressive types of skin cancers. Anti-angiogenic compounds can suppress melanoma cells. Orthosiphon stamineus (O.S) is a well-known herb with anticancer and anti-angiogenic effects. The health benefits of O.S extract is mainly due to its high antioxidant activity that can be attributed to rosmarinic acid (RA). However, RA has a limited ability to penetrate through the skin because of its hydrophilicity via topical application. The aim of this study is therefore to improve the penetrability of O.S extract through skin by sophorolipid (SL) nanovesicles, namely ethosome in mice. Methods Ethosome was made using thin film hydration method with sophorolipid as the surfactant. The ethosomal formulation of O.S (O.S-SL) was prepared at different concentrations and were physico-chemically characterized. Anti-angiogenic studies were then conducted. Anti-melanoma study was performed in albino mice using melanoma cell line (B16F10). Results The optimized vesicle was 387nm in size with -35.6mV potential and exhibited 10 times better permeation ability when compared to the hydroethanolic solution of O.S extract. O.S-SL revealed 100% anti-angiogenic effect ex vivo and 95.8% anti-melanoma effect in vivo. Conclusion The results of this study demonstrated that O.S-SL can be absorbed efficiently via topical application to the deeper layers of the skin. The formulation has shown promising anti-angiogenic and anti-melanoma effects after topical applications. Acknowledgements The authors would like to express their gratitude to Universiti Sains Malaysia, EMAN Biodiscoveries Sdn. Bhd. and NatureCeuticals Sdn. Bhd. for providing research facilities. Keywords: Orthosiphon stamineus, Ethosome, Sophorolipid, Melanoma, Topical Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Oral Presentation Topic: Cancer Citation: Nazari Vishkaei M, Khadeer Ahamed Basheer M and Malik Shah Abdul Majid A (2019). In vivo topical delivery of ethosomal formulation composed of Orthosiphon stamineus extract complexed with sophorolipid against melanoma. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00034 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 21 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Prof. Amin Malik Shah Abdul Majid, University of Science, Malaysia, George Town, Penang, 11800, Malaysia, aminmalikshah@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Mansoureh Nazari Vishkaei Mohamed Khadeer Ahamed Basheer Amin Malik Shah Abdul Majid Google Mansoureh Nazari Vishkaei Mohamed Khadeer Ahamed Basheer Amin Malik Shah Abdul Majid Google Scholar Mansoureh Nazari Vishkaei Mohamed Khadeer Ahamed Basheer Amin Malik Shah Abdul Majid PubMed Mansoureh Nazari Vishkaei Mohamed Khadeer Ahamed Basheer Amin Malik Shah Abdul Majid Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.