Orthosiphon stamineus improves disturbances of learning, recognition memory and spatial long-term memory in scopolamine-induced amnesia in rats

Abstract

Event Abstract Back to Event Orthosiphon stamineus improves disturbances of learning, recognition memory and spatial long-term memory in scopolamine-induced amnesia in rats Thaarvena Retinasamy1, Yatinesh Kumari1, Iekhsan Othman1 and Mohd. Farooq Shaikh1* 1 Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Neuropharmacology Research Laboratory, Malaysia Alzheimer's disease (AD) is a chronic neurodegenerative brain disease, characterized by impairment in cognitive functioning. Functional deficit in cholinergic system is believed to contribute to memory declineand characteristic feature of AD. There is pressing need to develop safer anti-AD therapies. Scopolamine, a muscarinic receptor antagonist, have profound amnesic effects in various learning paradigms in animal models. It is known to disrupt the cholinergic neurotransmission and involved in cognitive impairment observed in AD. There is a growing interest in the potential of phytochemicals to improve cognitive abilities. Rosmarinic acid, one of the main flavonoid components of Orthosiphon stamineus (OS) has been shown to have various pharmacological properties. In vitro studies, have demonstrated neuroprotective as well as acetylcholinesterase inhibitory effects of this compound. The present study, aimed at determining whether this Malaysian plant derived flavonoid rich OS extract can reverse scopolamine induced learning and memory dysfunction in the novel object recognition test and the elevated plus maze test. Rats in different groups were treated once daily with OS extract (100, 200, and 400mg/kg) and donepezil (1mg/kg) by oral route for 19 days where the last 8 days of treatment regimen included daily intraperitoneal (ip) injection of scopolamine 1mg/kg to induce cognitive deficits. Rats were subjected to behavioral analysis to assess learning and memory functions. The hippocampal tissues were extracted for gene expression analysis of BDNF, TrkB and CREB1, known to be modulated in AD condition. OS extract also exhibited memory-enhancing activity against scopolamine-induced memory deficits in the long-term memory novel object recognition performance as indicated by an increase in the discrimination index. Based on preliminary findings, it is found that the OS ethanolic extract helps in improving effect on memory and hence could serve as a potential therapeutic option for the treatment of neurodegenerative diseases like AD. Keywords: Amnesia, Memory, neurodegeneration, in vivo, Alzheimer’s disease Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: Poster Presentation Session Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Retinasamy T, Kumari Y, Othman I and Shaikh M (2016). Orthosiphon stamineus improves disturbances of learning, recognition memory and spatial long-term memory in scopolamine-induced amnesia in rats. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00189 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Dr. Mohd. Farooq Shaikh, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Neuropharmacology Research Laboratory, Bandar Sunway, Selangor, Malaysia, farooq.shaikh@monash.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Thaarvena Retinasamy Yatinesh Kumari Iekhsan Othman Mohd. Farooq Shaikh Google Thaarvena Retinasamy Yatinesh Kumari Iekhsan Othman Mohd. Farooq Shaikh Google Scholar Thaarvena Retinasamy Yatinesh Kumari Iekhsan Othman Mohd. Farooq Shaikh PubMed Thaarvena Retinasamy Yatinesh Kumari Iekhsan Othman Mohd. Farooq Shaikh Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.