Abstract
<h3>Purpose</h3> The objective was to present a method for the repeated noninvasive measurement of tumor oxygenation (Po<sub>2</sub>) over the whole period of tumor growth. <h3>Methods and materials</h3> A mixture of tumor homogenate (GH3 prolactinoma) and alginate capsules loaded with perfluoro-15-crown-5-ether (15C5) was injected into the flanks of Wistar Furth rats. The temporal behavior of tumor Po<sub>2</sub> was monitored between Day 1 and 26 after injection using fluorine-19 (<sup>19</sup>F) magnetic resonance imaging (MRI). In addition, the response of tumor Po<sub>2</sub> to modifiers of the tumor microenvironment (carbogen [95% O<sub>2</sub>/5% CO<sub>2</sub>], nicotinamide, and hydralazine) was investigated. <h3>Results</h3> An initial increase of tumor Po<sub>2</sub>, probably reflecting neovascularization, followed by a decrease after Week 2, probably indicating tumor hypoxia or necrosis, were observed. The minimum and maximum average Po<sub>2</sub> ± SEM observed were 3.3 ± 2.0 mm Hg on Day 2 and 25.7 ± 3.8 mm Hg on Day 13, respectively. Carbogen increased the tumor Po<sub>2</sub>, whereas nicotinamide caused no significant change and hydralazine induced a significant decrease in tumor oxygenation. <h3>Conclusions</h3> A preclinical method for the repeated noninvasive determination of tumor Po<sub>2</sub> was presented. It might help to investigate tumor physiology and the mechanisms of modifiers of the tumor microenvironment and their role in different therapeutic approaches.
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