Abstract
Extracts from Xenopus germinal vesicles have been used to transcribe faithfully cloned Drosophila or yeast tRNA genes to precursor tRNAs. If the tRNA genes contain an intervening DNA sequence it is found in the tRNA precursors as the corresponding RNA sequence. The Xenopus nuclear extracts have the ability to process these precursor tRNAs into mature tRNA. Sequence analysis of the primary transcripts, which contain 5′-terminal pppA or pppG residues, should aid studies aimed at defining regulatory DNA regions responsible for gene transcription by RNA polymerase III. In addition, these tRNA precursors have proven to be ideal substrates for the many enzymes involved in eukaryotic tRNA biosynthesis.
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