In - Vitro Study on Anti Fungal Activity of Various Potencies of Sepia Against Candida Albicans

Abstract

In this study , we have tested the ability of Sepia to limit the growth of a Candida culture. The current study's objective is to evaluate the effects of this drug on the fungus at various potencies. For almost two decades, homoeopaths have worked to establish the existence of the miraculous potential of little doses. The overall purpose of all of this research is to demonstrate, through in-vitro modifications, how Sepia acts on Candida fungus. The disc diffusion assay was used to evaluate the antifungal activity. Using a micropipette, a known volume of 10 μL of sepia of potency 200 C and 1M was loaded over small, circular, sterile paper discs (6mm in diameter) that had been impregnated in SDA plates. The plates were left at room temperature so that the sample could be absorbed into the medium before being incubated there for 24 to 48 hours. In order to assess the antifungal activity, the diameter of inhibition zone is measured. Itraconazole is used as positive control and ethanol as negative control.