In vitro study of the antioxidant activity of extracts from dried biomass of callus, cell suspension, and root cultures

Abstract

The most promising sources of antioxidants are plant objects that contain natural antioxidants such as polyphenols, carotenoids, and vitamins. The paper studies the antioxidant activity of extracts from dried biomass of callus, suspension cell cultures, and root cultures in vitro. The study of the antioxidant activity of plant extracts showed that the maximum antioxidant activity (250.6 mg AA/g) is possessed by extracts from the dried biomass of in vitro hyssop root cultures. According to this indicator, extracts from dried biomass of hyssop hairy exceed extracts from dried biomass of Amur maakia root cultures by 2.23 times, and extracts from dried biomass of Siler root cultures by 5.61 times. Extracts from the dried biomass of in vitro callus, cell suspension, and root cultures of Siler do not possess high antioxidant activity, which varies from 16.56 to 44.66 mg AA/g. The maximum indicator of antioxidant activity for this medicinal plant is observed in extracts from dried biomass of in vitro root cultures. For extracts from dried biomass of in vitro callus, cell suspension and root cultures of Amur maakia, the range of antioxidant activity indices is from 85.40 mg AA/g to 112.33 mg AA/g. The maximum antioxidant activity was observed in extracts from dried biomass of in vitro root cultures. A higher accumulation of anthocyanins was also found in the callus culture of Malus sieversii f. niedzwetzkyana cultured on a nutrient medium containing both auxins and cytokinins.