Abstract
In vitro regeneration of sugarcane varieties viz. Isd-20, Isd-30 and Isd-2/54 has been studied. Nutrient media containing growth regulators enhanced callus induction, shoot differentiation and root formation in vitro. All the three varieties cultured fortified with 3.0 mg/1 2,4-D, showed the best performance in the callus induction on modified MS media. In shoot induction, effectiveness of cytokinin (BA 1.5 mg/1) along with either 0.25 mg/1 NAA or IBA was proved to be superior to other concentrations. Microshoots were rooted on MS and modified MS media. The striking number of roots was observed on modified MS medium supplemented with NAA 0.5 mg/1 + 0.5 mg/1 IBA and IBA 0.5 mg/1 + LAA 0.5 mg/1. Eighty to ninety per cent regenerated plantlets were viable with 85% relative humidity while transferred to sterilized soil.
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