Abstract
In vitro regeneration of sugarcane varieties viz. Isd-20, Isd-30 and Isd-2/54 has been studied. Nutrient media containing growth regulators enhanced callus induction, shoot differentiation and root formation in vitro. All the three varieties cultured fortified with 3.0 mg/1 2,4-D, showed the best performance in the callus induction on modified MS media. In shoot induction, effectiveness of cytokinin (BA 1.5 mg/1) along with either 0.25 mg/1 NAA or IBA was proved to be superior to other concentrations. Microshoots were rooted on MS and modified MS media. The striking number of roots was observed on modified MS medium supplemented with NAA 0.5 mg/1 + 0.5 mg/1 IBA and IBA 0.5 mg/1 + LAA 0.5 mg/1. Eighty to ninety per cent regenerated plantlets were viable with 85% relative humidity while transferred to sterilized soil.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.