In Vitro Propagation of White Stokesia(Stokesia laevis Greene var. alba hort.) by Leaf and Root Culture.

Abstract

In vitro propagation of white stokesia (Stokesia laevis Greene var. alba hort.) was studied with leaf- and root-section cultures. Shoot apices cultured on Murashige-Skoog (MS) medium supplemented with 1 ppm N6-benzylaminopurine (BA) produced multiple shoots. These shoots rooted well on one-fifth concentration of MS medium (major elements only) with 1 ppm 3-indolebutyric acid (IBA). Excised leaf and root sections were cultured on the MS media containing 0, 0.1, 1.0 or 5 ppm BA and 0, 0.1, 1.0 ppm α-naphthaleneacetic acid (NAA). In leaf section culture, high percentages of adventitious buds were obtained; the maximum number of bud per leaf section was 12.8 on the medium with 5 ppm BA plus 0.1 ppm NAA. In root section culture, adventitious bud formation rate was relatively low, compared to leaf. section culture. A maximum number of adventitious bud per root section was 6.0 on the medium with 1 ppm BA. Rooted plantlets were successfully acclimatized when transplanted to the soil and cultured in a greenhouse. In vitro propagation with leaf section of white stokesia is more efficient than the conventional shoot division or root-cutting.