In vitro Plantlet Regeneration from Leaf and Stem Calluses of Rauwolfia tetraphylla (R. canescens) and Confirmation of Genetic Fidelity of Plantlets Using the ISSR-PCR Method

Abstract

Leaf and stem explants of Rauwolfia tetraphylla (syn.R. canescens), an alternative of endangered medicinal plant, R. serpentina cultured on MS + 2,4-D (2.26 μM.L−1) + Kn (9.29 μM.L−1) produced green and compact callus after 4 weeks of culture. When these primary calluses were sub-cultured successively for 3 passages, they became light green and fragile and when they were grown on MS + TDZ (2.27 μM.L−1), they produced highest no of multiple shoot buds. Individual microshoots were successfully rooted on MS+IAA (5.37 μM.L−1), and 88% of the plantlets survived in field conditions. ISSR-specific primer HY3 banding profile of the plantlets are similar to the mother plant.