NAA或2,4-D組合Thidiazuron對山煙草癒傷組織誘導與增殖之影響

Abstract

In this study, the combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthalene-1-acetic acid (NAA) (0, 1.25, 2.5, 5 mg L^(-1)) and 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (TDZ) (0, 0.1, 0.5, 1 mg L^(-1)) were used to test their effects on callus formation from explants of leaf, stem and root. Three weeks after culture, the explants of leaf and stem gave a higher callus formation rate than that of root. The developed calli showed two different morphogy, the pale green and compact calli or the yellowish white and friable calli. The calli from primary as secondary explants were used for further experiment of callus proliferation. Calli proliferation was better on MS basal medium containing 2 mg L^(-1) 2,4-D or auxin-free medium. The calli are suitable phytomaterials applied to the suspension culture of cells or adventitious roots, which can be used to isolate and purify bioactive steroidal alkaloids.