Abstract
The Cell cultures are used extensively for in vitro secondary metabolite productions were obtained from callus tissue through cell suspension culture. The establishment of callus cultures has considerable potential for the production of known and novel secondary metabolites. The objective of the study was to scientifically assess callus culture of Biophytum sensitivum (L) DC. was established from leaf explants with different growth regulators greatly influenced the growth of callus cultures. The callus from leaf explants is induced by inoculating the young leaf bits on MS medium supplemented with various auxins (2, 4- Dichlorophenoxyacetic acid (2, 4-D), ?-Naphthalene Acetic Acid (NAA) and Indole Buteric Acid (IBA), cytokinins (6-Benzyladenine (BA) and Kinetin (KN) and cytokinin-auxin combination (BA+NAA) in different concentrations were (0.5 to 5.0 mg/l) used. BA 1mg/l, in combination with NAA (1.0 mg/l) also produced maximum amount of callus. So, this research is concluded that the plant leaf explants cultured on MS medium with 1 mg/ l BA with 0.5 mg/l NAA was found most efficient for callus induction, provided calli with quite good in texture and friable in nature.
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