Abstract
An efficient plant regeneration system was developed for Hordeum vulgare L. cv Baegdong - an important high yielding Korean cultivar. This cultivar is resistant to Fusarium and a few other fungi but susceptible to Barley Yellow Mosaic Virus (BaYMV) and Barley Mild Mosaic Virus (BaMMV). The protocol was based on a series of experiments involving the sizes of immature embryos and the culture media. The embryo size is found to be critical for the establishment of callus. Embryos of 1.6-2.0 mm size showed the highest ability to produce callus capable of regenerating green plants. The auxins picloram and dicamba proved effective in inducing callus from immature embryos. 2.5 mg L -1 dicamba and 4.0 mg L -1 picloram in Murashige and Skoog (MS) medium was optimum for the induction of primary callus. The induced primary callus was loose and friable which ultimately developed into creamy white and compact callus after transferring into the fresh medium. Multiple shoots were induced in the ViMS medium supplemented with 6.0 g L -1 maltose, 20.0 g L -1 sorbitol, 0.5 mg L -1 2, 4-D and 1.0 mg L -1 kinetin and the rate was 6.5 shoots per embryo. Regenerated plants were hardy and developed roots rapidly in the medium containing 0.2 mg L -1 Indolebutyric acid (IBA). This efficient plant regeneration system provides a foundation for generating transgenic plants of this important barley cultivar. Keywords: Barley embryo, embryo size, Hordeum vulgare, plant regeneration, primary callus Normal 0 false false false MicrosoftInternetExplorer4 J. Natn.Sci.Foundation Sri Lanka 2007 35(3): 161-165 doi: 10.4038/jnsfsr.v35i3.2014
Highlights
Barley (Hordeum vulgare L.) is an important cereal crop used for malting, brewing, distilling and as an animal as well as a human food
Genetic transformation is a key tool for genetics research which can be of agronomic importance
Corresponding author of generating transgenic barley. Both biolistics and Agrobacterium-mediated methods have been successfully applied for genetic transformation of barley, most work has been performed on a single model barley cultivar, 'Golden Promise'[13]
Summary
Barley (Hordeum vulgare L.) is an important cereal crop used for malting, brewing, distilling and as an animal as well as a human food. Barley has been the subject of extensive genomics research and is considered a model for the Family Triticeae. Both biolistics and Agrobacterium-mediated methods have been successfully applied for genetic transformation of barley, most work has been performed on a single model barley cultivar, 'Golden Promise'[13]. Immature embryos are presently being used as the explant[14] and many related factors such as the size of the embryo, and growth regulator combinations involved in successful regeneration have been studied in detail. Immature embryos are suitable explants for genetic transformation but this requires a consistent supply of stock barley plants in growth chambers or greenhouses. The above protocol has a poor success rate when applied to other barley cultivars. Selecting the proper stage of maturity may be one of the limiting factors when using immature embryos
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