Abstract
A reliable protocol for callus induction and regeneration were developed for leaf explants of Artemisia vulgaris L. Percent of callus induction and regeneration were higher in the young leaves. MS medium containing 1.0 mgl -1 BAP and 3.0 mgl -1 NAA is the optimum concentration for induction of callus. So produced callus was subcultured on Murashige – Skoog (MS) medium with 1.0 mgl -1 6-benzylaminopurine (BAP), 3.0 mgl -1 gibberellic acid (GA 3 ) produced the highest mean number of shoots (35.85±0.81) per explant. Half strength of MS was found to be the best for rooting, however, addition of IAA (0.5-1.0 mgl -1 ) was found essential to induce longer roots. More than 84% of the rooted plants were established in polycups after hardening.
Highlights
Artemisia vulgaris Linn, an important perennial medicinal herb belongs to the family Asteraceae
The present study describes an optimized regeneration system in Artemisia vulgaris callus derived from leaf explant cultures on a variety of medium composition
The leaf segments were washed with 5% (w/v) bavistin, 10% (w/v) antibiotic and 5% (w/v) Tween 20 by continuous shaking for 20 min followed by washing in tap water, rinsed three to five times with double distilled water
Summary
Artemisia vulgaris Linn, an important perennial medicinal herb belongs to the family Asteraceae. The plant is aromatic, shrubby 0.6-2.4m high and pubescent. The plant has a hot, sharp and pungent taste. The leaves and shoot tips are administered in nervous and spasmodic affections connected with debility, in asthma and diseases of the brain. It shows antispasmodic and anthelmintic activity(Kirtiker and Basu, 1935). The present study describes an optimized regeneration system in Artemisia vulgaris callus derived from leaf explant cultures on a variety of medium composition
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