Abstract
Summary A droplet-freezing method has been developed for the cryopreservation of cassava, Manihot esculenta Crantz, meristems. The meristems treated with a 15 % DMSO + 3% sucrose solution for 15 min were frozen over an 18 μm aluminium foil in 2–3 μl droplets of the cryoprotectants in plastic petri dishes at a cooling rate of 0.5°C/min to various sub-zero temperatures (-20; -25; -30 and -40°C) and stored in liquid nitrogen (-196°C). The meristems retrieved from liquid nitrogen storage, upon thawing and return to in vitro culture on plant regeneration medium, exhibited various morphogenetic responses such as differentiation of callus and leaves and whole plantlets. The plantlets were successfully grown in pots.
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