Abstract
In order to establish the plant regeneration system of Glycyrrhiza mongolicum and Glycyrrhiza Xinjiang, this study used hypocotyls, leaves, cotyledons and stems to inducing callus and regenerated plant, and it screen out the most suitable explants and the best medium for plant regeneration. The experimental results showed that MS+2.0 mg/L NAA+2.0 mg/L 6-BA was the best medium for callus induction and plant regeneration. Among these, hypocotyls and cotyledons are the best explants for callus induction. The tissue induction rate is higher than 90%. The rapid propagation system established in this study can provide the theoretical reference and practical guidance for the breeding and production of fine Glycyrrhiza uralensis Fisch. varieties.
Highlights
Glycyrrhiza uralensis Fisch. is a plant of the genus Glycyrrhiza in the leguminous family, with the reputation of "ten directions and nine herbs"
1.2 Callus induction rate of different tissues of G. mongolicum The hypocotyls, leaves, cotyledons and stems of G. mongolicum were cultured in callus induction medium with different hormone ratios (NAA and 6-BA) (Figure 2)
The results show (Table 2) that the induction rate of stem and hypocotyl was higher in MS+0.5 mg/L NAA+0.5 mg/L 6-BA hormone induction medium; the induction rate of cotyledon and hypocotyl was higher in MS+1.0 mg/L NAA+1.0 mg/L 6-BA and MS+2.0 mg/L NAA+2.0 mg/L 6-BA hormone induction medium, and the callus formed by cotyledon and hypocotyl grew well
Summary
Glycyrrhiza uralensis Fisch. is a plant of the genus Glycyrrhiza in the leguminous family, with the reputation of "ten directions and nine herbs". The use of tissue culture technology to establish a rapid propagation system of G. uralensis is beneficial to increase the propagation speed of G. uralensis, the extraction of effective ingredients and the preservation of good varieties (Li et al, 2013). The tissue culture and rapid propagation system of Glycyrrhiza glabra L. have not been reported, so this study further optimized the aseptic and rapid propagation system of G. glabra in the local areas (Inner Mongolia and Xinjiang) based on the previous research on the rapid propagation of G. uralensis tissues, in order to speed up the G. uralensis sterile breeding, maintain good varieties of medicinal properties, promote the industrialization development of G. uralensis, and provide the theoretical basis for the breeding efficiency of G. uralensis and the preservation, development and utilization of the superior varieties
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