Abstract
The effect of potassium dihydrogen phosphate (KH 2 PO 4 ) on callus induction and plant regeneration from callus cultures of indica rice ( Oryza sativa cultivar Pusa Basmati-1) was investigated. Callus was induced on Murashige and Skoog (MS) medium supplemented with 11.31 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and subsequent plant regeneration was obtained on MS medium containing 2.68 μM α-naphthaleneacetic acid (NAA) and 8.87 μM 6-benzylaminopurine (BAP). Both the induction and the regeneration media were supplemented with different levels of KH 2 PO 4 (0 to 12.50 mM). The level of KH 2 PO 4 in the induction medium influenced the percent water content of the callus. KH 2 PO 4 was found to be essential for plant regeneration as no shoot regeneration occurred in the absence of KH 2 PO 4 . Highest number of shoots per explant was obtained when KH 2 PO 4 level in the callus induction and plant regeneration medium was 0.625 mM (half of MS level) and 1.25 mM (normal MS level) respectively. By making amendme...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
