Abstract
Although the immunomodulatory effects of vitamins, minerals, and many herbs per se have been extensively studied, while research related to possible immunomodulatory effects of a combined formulation is relatively scarce. Here, the potential immunomodulatory effects of the combination of eight components viz. zinc chloride, ferrous sulfate, sodium selenate, nanocurcumin, copper chloride, magnesium gluconate, vitamin C, and vitamin D3 were investigated. These components are widely used in therapeutic, cosmetics and dietary supplements. The current study investigated the in vitro immunomodulatory effect of a Consciousness Energy Healing (The Trivedi Effect®) Treated nanocurcumin-based test formulation in lipopolysaccharide (LPS)-induced natural killer (NK) cells activity in splenocytes cell-line co-incubated with mouse lymphoma cell line (Yac-1). The formulation was divided into two parts, in which one part of the formulation was received the Biofield Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi and was defined as the Biofield Energy Treated sample, while the other part was denoted as the untreated test sample. Cell viability using MTT assay in mouse splenocytes showed more than 98% cells were viable upto 10.4 µg/mL of the tested concentrations, indicating that the test formulation was safe and nontoxic. The NK cells activity was measured in cell supernatants using ELISA. The NK cells activity was significantly altered by 26.73%, 11.82%, 7.64%, 0.36% and 36.36% in the untreated formulation group at 0.1, 0.5, 1, 5.2, and 10.4 µg/mL, respectively compared to the vehicle control (VC) group. Further, Biofield Energy Treated test formulation exhibited 39.64%, 8.18%, 17.27%, 51.93% and 42.55% alteration of the NK cells activity at the concentrations of 0.1, 0.5, 1, 5.2, and 10.4 µg/mL, respectively compared to the VC group. Moreover, the cell viability of the test formulation was assessed in RAW 264.7 (Mouse Macrophage Cell Line) for phagocytosis activity was safe upto 13.3 µg/mL with >90% on cell viability. The phagocytosis assay data showed that at two concentrations (1.3 and 7.6 µg/mL) the Biofield Energy Treated test formulation improved extent of phagocytosis by 6.31% and 5.91%, respectively compared to the untreated test formulation group. In summary, the Biofield Energy Treated test formulation may have indeed immunomodulatory effect by enhanced NK cells activity and extent of phagocytosis responses. These observations indicated that the Biofield Energy Treated test formulation has the potential effects through modulating the expression of NK cells function and phagocytosis and might be developed as a useful anti-inflammatory product for various inflammatory disorders.
Highlights
With the concern of defense capability against infectious microbes, innate immunity evokes preexisting defense mechanisms that have specialized to recognize microbes and eliminate infection [1]
At two concentrations (1.3 and 6.7 μg/mL) the Biofield Energy Treated test formulation improved extent of phagocytosis by 6.31% and 5.91%, respectively compared to the untreated test formulation group
The cell viability of the nanocurcumin-based test formulation was found as safe at the tested concentrations upto 10.4 μg/mL in mouse splenocytes
Summary
With the concern of defense capability against infectious microbes, innate immunity evokes preexisting defense mechanisms that have specialized to recognize microbes and eliminate infection [1]. Positive outcomes in different disease profiles [19] This subtle form of energy can be harnessed and transmitted by individuals into living and inanimate object via the process of unique Energy Transmission process (The Trivedi Effect®) and the data has been published in numerous peer-reviewed science journals with significant outcomes in cancer research, microbiology, genetics, pharmaceutical science, agricultural science, and materials science [20-37]. In this regards, authors intend to evaluate the impact of Biofield Energy Healing Treatment (The Trivedi Effect®) on the herbomineralbased formulation for immunomodulatory action in two different cell-lines (splenocytes co-incubated with Yac-1 and macrophage) with the estimation of NK cells activity and phagocytosis responses
Sign-in/Register to view highlights & summary 
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call