Abstract
Sleep biomarkers in brain such as melatonin, BDNF (Brain-derived neurotrophic factor), PGD2 (Prostaglandin D2), leptin, orexin-A, and acetylcholine were evaluated in the unpredictable chronic stress (UCS) rodent model in the presence of Consciousness Energy Healing Treated (the Trivedi Effect®) novel test formulation in male Sprague Dawley (SD) rats using ELISA assay. The test formulation was consisted of minerals (Zn, Fe, Cu, Se, Ca, Mg), vitamins (C, E, B6, B12, D3), β-carotene, ginseng, and cannabidiol isolate. The test formulation constituents were divided into two parts, one part of each ingredient was distinct as the untreated test formulation, while the other portion of the test formulation and a group of animals received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of melatonin in groups viz. G5 (Biofield Energy Treated Test formulation) and G7 (15-days pre-treatment of Biofield Energy Treated Test formulation) was significantly increased by 17.6% (p≤0.01) and 16%, respectively as compared with the disease control group (G2). Brain-derived neurotrophic factor (BDNF) level in brain was increased by 5.2% in G7 group as compared with the G4. Prostaglandins D2 (PGD2) level was significantly (p≤0.001) increased by 12.7%, 18.1%, 23.7%, and 30.7% in the G6, G7, G8 (15 days pre-treatment of Biofield Energy Treated Test formulation to the Biofield Energy Treatment per se rats), and G9 (untreated test formulation to the Biofield Energy Treatment per se to the rats) groups, respectively as compared with the G2. The level of leptin after Biofield Energy Treatment and with the test formulation was altered. However, orexin-A level was significantly decreased by 37.1% (p≤0.05), 32.6%, 40.5% (p≤0.05), 44.4% (p≤0.05), and 28.2% in the G5, G6, G7, G8, and G9 groups respectively, as compared with the G2. Similarly, acetylcholine (Ach) level was significantly (p≤0.001) decreased by 42.5%, 49.2%, 40.1%, 47.9%, and 45% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2. Overall, the results showed the significant slowdown the stress-related disease progression and its complications/symptoms in the preventive in the Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease group. The Trivedi Effect® showed increased level of melatonin and decreased levels of insomnia related brain biomarkers which might be helpful to induce better sleep in human.
Highlights
Stress could be considered as a nonspecific and adaptive response that may occur due to some social environmental factors
The data showed the melatonin level in the unpredictable chronic stress (G2) group was 75.31 ± 4.1 pg/mL, which was significantly decreased by 30.9% as compared with the normal control (G1, 109.04 ± 3.4 pg/mL)
Melatonin level in brain was measured and reported to be significantly increased by 17.6% (p≤0.01) and 16% in the G5 (Biofield Energy Treated Test formulation to the untreated rats) and G7 (15-days pre-treatment of Biofield Energy Treated Test formulation), respectively as compared with the disease control group (G2)
Summary
Stress could be considered as a nonspecific and adaptive response that may occur due to some social environmental factors. It may induce some kind of mental disorders, in which the patients exhibited cognitive problems and persistent fatigue such as lack of concentration and memory loss. The research studies showed that chronic stress may impair the early long-term potentiation (LTP) in the at-risk rat model [9], and may increase amyloid-β plaque deposition as well as decrease the hippocampal synaptic plasticity in Tg2576 mice [10, 11]. Some researchers confirmed that chronic stress exposure may increase the glucocorticoids levels, neuronal injury, promoted senile plaque deposition, and cognitive impairment in mice models [12]
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