In Vitro Assessment of Estrogenic Potential of Biofield Energy Treatment using Human Endometrial Adenocarcinoma Cell Line

Mahendra Kumar Trivedi,Snehasis Jana,Manjubala Dash

doi:10.14302/issn.2381-862x.jwrh-18-2459

Abstract

The objective of the study was to investigate the effect of Consciousness Energy Healing based DMEM medium on the level of alkaline phosphatase enzyme (ALP) activity in Ishikawa cells. The test item, DMEM medium was divided into two parts. One part of the test item received Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Mahendra Kumar Trivedi and was labeled as the Biofield Energy Treated DMEM, while the other part did not receive any treatment, and defined as the untreated DMEM group. The cell viability using MTT assay of the Biofield Energy Treated DMEM group was observed as 108%, which indicated that the test item was safe and non-toxic. The estrogenic potential using ALP level showed a significantly increase by 73.21% in the Biofield Energy Treated DMEM group as compared to the untreated DMEM group. Overall, the experimental data suggested that the Biofield Energy Treated DMEM has significantly improved ALP level, which play a vital role for the promotion and maintenance of estrogen level. Based on the study outcomes, it is concluded that Biofield Energy Healing Treatment showed a significant improved ALP level, which can be used in various estrogenic disorders such as hypophosphatasia, osteoporosis, severe anemia, malnutrition, hypothyroidism, magnesium deficiency, heart surgery, aplastic anemia, chronic myelogenous leukemia, enteritis in children, Wilson’s disease, pernicious anemia, bacterial infection and intrauterine infection is a leading cause of pelvic inflammatory disease, subfertility, infertility, endometritis, early pregnancy loss, fetal defects, and preterm birth.

Highlights

Alkaline phosphatase (ALP) activity is found in high concentration in the endometrium region of menstruating women
On the basis of Biofield Energy Treatment outcome, authors in this study evaluates the impact of the Biofield Energy Healing Treatment (The Trivedi Effect®) on DMEM as test sample for estrogenic potential with respect to ALP parameter using standard in vitro assay in Ishikawa cells
Biofield Energy Treatment (The Trivedi Effect®) on DMEM was studied to evaluate the estrogenic potential and in vitro results showed a significant improvement with respect to ALP activity

Summary

Introduction

Alkaline phosphatase (ALP) activity is found in high concentration in the endometrium region of menstruating women. Alkaline phosphatases (ALPs) are the zinc-containing metalloenzymes plasma membrane-bound glycoproteins, which are widely distributed in nature [3]. They functions as dimeric molecules and encoded by a multigene family and have three metal ions including two Zn2+ and one Mg2+ in the active site for their essential enzymatic activity [4]. The altered activity pattern of ALP is regulated by the sex hormones, along with the epithelial alkaline-phosphatase (e-AP) concentration, which is on peak at maximum estrogen stimulation. It was reported that most rapid endometrial growth happens at the time of maximum ALP concentration, which regulates the proper endometrial function. Environment have various factors such as natural or synthetic, which have significant potential to inhibit the estrogen action and causes its functional disruption [5]

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