Abstract
Erectile dysfunction is a common disorder found in men, which occurs due to multiple factors such as psychogenic, hormonal imbalance, and neurovascular disturbances. The present investigation was undertaken to examine the effect of a Consciousness Energy Healing based DMEM medium on the Human Endothelial Hybrid Cell Line (EA. hy926) to evaluate the level of cyclic guanosine monophosphate (cGMP). The test item (DMEM medium) was divided into three parts, first part received a one-time Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Alice Branton and was labeled as the one-time Biofield Energy Treated (BT-I) DMEM, while second part received the two-times the Biofield Energy Treatment and is denoted as BT-II DMEM. The third part did not receive any treatment and defined as the untreated DMEM group. The level of cGMP for the inhibition of PDE-5 enzyme was assessed using cGMP ELISA assay kit (colorimetric). Sildenafil citrate, used as positive control, which showed a significant increase of cGMP levels in Ea. hy926 cells. The one-time Biofield Energy Healing Treated DMEM (BT-I) showed 29.92% alteration, while the two-times Biofield Energy Healing Treated DMEM (BT-II) showed a significantly (p≤0.001) increased level of intracellular cGMP by 307.09% in Ea. hy926 cells compared to the untreated DMEM group. Overall, experimental data suggested that the two-times Biofield Treated DMEM showed a significantly improved level of cGMP compared with the one-time Biofield Treated DMEM group. Therefore, data indicated that the Biofield Energy Healing Treatment can be used to treat erectile dysfunction along with other sexual disorders such as orgasmic disorders, female sexual arousal disorder, fetishistic disorder, frotteuristic disorder, hypoactive sexual desire disorder, sex addiction, sexual masochism and sadism, vaginismus, voyeuristic disorder, premature or delayed ejaculation.
Highlights
More than 30 million men in the United States and far more all over the world have erectile dysfunction (ED), which is a pervasive disorder and a most common disability
From outstanding benefits of Biofield Energy Treatment, the present study aimed to evaluate the impact of the Biofield Energy Treated (The Trivedi Effect®) Dulbecco’s Modified Eagle’s Medium (DMEM) on ED using the standard in vitro assay in Human Endothelial Hybrid Cell Line (EA. hy926) cells
The one-time Biofield Energy Treated test item (BT-I) and the untreated DMEM showed almost a similar concentration of cyclic guanosine monophosphate (cGMP) (0.89pmol/mL), while the two-times Biofield Energy Treated group (BT-II) showed a significant increase the level of intracellular cGMP concentration by 307.09% compared to the untreated DMEM group
Summary
More than 30 million men in the United States and far more all over the world have erectile dysfunction (ED), which is a pervasive disorder and a most common disability. Neurogenic, hormonal, and psychological factors play an important role for a penile erection or its dysfunction [3]. Impaired function of arteries and corpora cavernosa within penis are the major condition for impotence, while lack of smooth muscle tone and imperfections in neuronal stimuli can lead to unsuccessful penile erection [4]. Nitric oxide synthase (NOS) enzymes play the significant role in the mechanism of ED. It enhances the production of cyclic guanosine monophosphate (cGMP), which results in smooth muscle relaxation and vasodilation via NO/cGMP pathway [7,8]. Some alternative or complementary therapeutic approach is very much required to treat impotence without any side-effects
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