Abstract

Oxidative stress is one of the major molecular mechanism responsible for human lung diseases. The objective of the study was to examine the effect of a Consciousness Energy Healing (The Trivedi Effect®) Treated DMEM medium for its anti-oxidative potential using various parameters such as the protection against oxidative stress and Superoxide Dismutase (SOD), antioxidant enzyme activity in A549 cells. The test item, DMEM was divided into two parts. One part received the Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Mahendra Kumar Trivedi and was labeled as the Biofield Energy Treated test item, while the other part did not receive any sort of treatment and is defined as the untreated DMEM group. The cell viability of the test sample using MTT assay showed 122.67% viable cells, indicating a safe and nontoxic profile of the test item. Protection against oxidative stress was significantly increased by 64.70% in the Biofield Energy Treated DMEM group compared to the untreated DMEM group. In addition, Antioxidant Enzyme (SOD) activity was significantly increased by 45.1% in the Biofield Energy Treated DMEM group compared to the untreated DMEM group. Thus, these data suggest that Biofield Energy Healing Treatment showed a significant improvement of the Antioxidant Enzyme Activity (SOD level) along with an improved protection against oxidative damage, which can be used in various human lung disorders such as asthma, Chronic Obstructive Pulmonary Disease (COPD), lung malignancies and parenchymal lung diseases like idiopathic pulmonary fibrosis and lung granulomatous diseases.

Highlights

  • Increased level of Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS) leads to oxidative stress, which is an imbalance between the oxidants and antioxidants

  • The Biofield Energy Healing Treated (The Trivedi Effect®) DMEM showed a significant improvement of cellular protection against oxidative stress and increased the antioxidant enzyme capacity with respect to Superoxide Dismutase (SOD) level in human lung cells

  • The antioxidative protection level against oxidative stress was significantly increased by 64.70% in the Biofield Energy Treated DMEM group compared with the untreated DMEM group

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Summary

Introduction

Increased level of Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS) leads to oxidative stress, which is an imbalance between the oxidants and antioxidants. This imbalance and increased ROS can directly affect the lipids, DNA, carbohydrates, and proteins [1] in the human cells. Lung is one of the major organs, which is highly exposed by various oxidants i.e. endogenous and exogenous oxidants (cigarette smoke, mineral dust, ozone, and radiation). These oxidants produced free radicals, while ROS and RNS are produced by phagocytes as well as by alveolar, polymorphonuclear, bronchial and endothelial cells [4]. The reduced level of the antioxidants and increased level of oxidants results in idiopathic pulmonary fibrosis and its associated lung diseases

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