In Vitro and In Vivo Biological Activities of Cissus adnata (Roxb.).

Mohammed Shoibe,Morshed Alam,Md Nazim Uddin Chy,Shababa Wajida Nihar,Nishat Rahman,Ehsan Suez,Md Zobidul Islam,Morshed Alam,Md Adnan

doi:10.3390/biomedicines5040063

Abstract

This study was conducted to evaluate the in vitro polyphenol content, antioxidant, cytotoxic, antibacterial, anthelmintic properties, and in vivo antinociceptive activity of the ethanol extract of Cissus adnata leaves (EECA) in different experimental models. Polyphenol contents were investigated using spectrophotometric techniques. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) radical-scavenging, ferric reducing power, and total antioxidant capacity assays. Cytotoxicity was determined by brine shrimp lethality bioassay and disc diffusion method was used for the antibacterial activity. Anthelmintic activity was studied using aquarium worm (Tubifex tubifex) whereas antinociceptive activity was evaluated in mice by acetic acid and formalin test. Phytochemical screening of EECA revealed the presence of alkaloids, carbohydrates, flavonoids, phenols, terpenoids, saponins, and tannins. EECA showed strong antioxidant activity with high polyphenol contents. It was observed that EECA possessed significant antibacterial activity with a low toxicity profile. EECA also demonstrated dose-dependent and statistically significant anthelmintic and antinociceptive activities. Our study shows that ethanol extract of C. adnata leaves possess strong antioxidant, antibacterial, anthelmintic and antinociceptive activities with lower toxicity. Further studies are needed to identify bioactive phytomolecules and to understand the mechanism of such actions better.

Highlights

Cissus adnata (Roxb.), a member of Vitaceae family, is a deciduous, large woody climber with yellow flowers, popularly known as Isswarmuli (Chakma), Mach toi kitab (Tripura), Alianga lota and Bhatia lota (Bengali)
The total flavonols content of EECA was expressed in Quercetin (QE), and the result was represented in Table 2 where the total flavonols content was 19.35 ± 1.16 mg quercetin equivalent (QE)/g dried plant extract
Acute toxicity occurrence of death or abnormal saponins, and tannins. the the study acute showed toxicity no study showed no occurrence of behavior death or up to a mg/kg dose for EECA, which indicates that the extract possesses a low toxicity profile. a abnormal behavior up to a 2000 mg/kg dose for EECA, which indicates that the extract possesses

Summary

Introduction

Cissus adnata (Roxb.), a member of Vitaceae family, is a deciduous, large woody climber with yellow flowers, popularly known as Isswarmuli (Chakma), Mach toi kitab (Tripura), Alianga lota and Bhatia lota (Bengali). It is widely distributed and naturally grown at Khagrachari, Bandarban, Cox’s Bazar, Sylhet and Chittagong regions in Bangladesh [1]. Different parts of this plant are used in traditional medicine to treat a variety of diseases and disorders. The plant is beneficial to treat scurvy, cancer, hemorrhoids, dysentery, epilepsy, fever, asthma, malaria, and food poisoning [2,3]

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