In vitro adhesion of Bacillus sp. and Enterobacter sp. probiotics on intestinal epithelial cells of red tilapia (Oreochromis sp.) and the application effects on the fish growth and survival rate

Abstract

This research aimed to determine the adhesion of Bacillus sp. (PCP1) and Enterobacter sp. (JC10) on intestinal epithelial cells of red tilapia (Oreochromis sp.) and the effect of the probiotics application in feed on fish growth, survival rate, and feed conversion ratio. In vitro adhesion test was performed by using 108 cells/ml of bacteria, and 105 cells/ml of epithelial cells for 1 hour of incubation. The probiotics were added to the fish pellet with the dose of 5 x 104 CFU/g of feed with four treatments, including probiotic application every three days, seven days, without probiotic, and commercial probiotic application every three days. Each treatment consists of three replications. Red tilapia is maintained for 30 days in fiberglass ponds. The feed is given two times per day with a dose of 5 % of the biomass. The adhesion experiment results showed that Bacillus sp. (PCP1) and Enterobacter sp. (JC10) have adherence abilities higher than the commercial probiotics. The application of probiotics in tilapia for one month did not affect the fish growth, survival rate, and feed conversion ratio (P > 0.05). Probiotic application in a longer period is needed to be addressed.