Abstract
The objective of this experiment was to evaluate estimates of concentration of indigestible dry matter (iDM), indigestible neutral detergent fiber (iNDF) and indigestible acid detergent fiber (iADF) in samples of cattle feed and feces processed at different particle sizes (1-, 2-, and 3-mm) using bags made from nylon (50 μm), F57 (Ankom®), and non-woven textile (NWT - 100 g/m²) in a in situ evaluation procedure. Eight samples of concentrates, seven samples of forages and two samples of cattle feces were used. The samples were put in the bags according to a ratio of 20 mg of dry matter/cm² of surface by using three bags of each textile for each size of particle, totaling 405 bags, which were submitted to ruminal incubation for 264 hours. The contents of indigestible compounds in the samples were evaluated sequentially. Blank bags were used for microscopic evaluation of textile physical integrity and for evaluation of the physical resistance. The presence of contaminants caused high variability of results of iDM, indicating that its using as internal marker should not be recommended. By using the textiles F57 and NWT, similar estimates for contents of iNDF and iADF were obtained whereas by using nylon (50 μm), the concentration of iNDF was underestimated, possibly because of loss of particles due to its porosity. Nylon presented loss of resistance in all analytical stages, expanding its probality of rupture, especially after extraction with acid detergent. For in situ evaluation of indigestible compounds, it is suggested grinding samples in 2-mm screen sieves.
Highlights
Internal markers are inherent components of feeds that can be used to estimate digesta flow and fecal mass in digestion assays with ruminant animals
The objective of this experiment was to evaluate estimates of concentration of indigestible dry matter, indigestible neutral detergent fiber and indigestible acid detergent fiber in samples of cattle feed and feces processed at different particle sizes (1, 2, and 3-mm) using bags made from nylon (50 μm), F57 (Ankom®), and nonwoven textile (NWT – 100 g/m2) in a in situ evaluation procedure
The objective of this work was to evaluate the contents of indigestible dry matter (iDM), indigestible neutral detergent fiber (iNDF) and indigestible acid detergent fiber (iADF) in samples of feeds and cattle feces processed at different particle sizes and using bags made from nylon (50 μm), F57 and NWT through in situ procedures
Summary
Internal markers are inherent components of feeds that can be used to estimate digesta flow and fecal mass in digestion assays with ruminant animals. The capacity to be completely recovered in feces or any segment of gastrointestinal tract is one of the demanded characteristics for an ideal marker (Owens & Hanson, 1992). Under the lack of such characteristic, the estimates of fecal excretion or digesta flow could be biased. When internal markers contents are evaluated using in situ procedures, different textiles can be used to make incubation bags. Among those, it can be highlighted the nylon (50 μm), the F57 (Ankom®) and the non-woven textile (NWT – 100 g/m2). Nylon could be assumed as standard textile for rumen incubation procedures. Controversial results have been reported with regard to its efficiency for obtaining contents of indigestible compounds, which seem to be associated with significant loss of particles (Casali et al, 2009)
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