Abstract
Summary Peroxidase-labelled Helix pomatia lectin (HPL), contrasted by histological counterstaining, enables a phenotypic and morphological differentiation of lymphocytes in formaldehyde- fixed tissues and cell smears. The HPL receptor was found on 71·5% of peripheral lymphocytes; granulocytes, macrophages and monocytes were negative. Double incubation assays with monoclonal antibodies revealed 97·1±2% of E-rosette+ cells were HPL+ and 90·3±3·5% of HPL+ lymphocytes were simultaneously OKT3+. In cytolysis studies, 8·67plusmn;4·1% of the cells were HPL+ and anti-human Lyt3−, and 5·8±13% of peripheral blood lymphocytes were HPL+ and OKT3. In human lymph nodes, the majority of HPL+ lymphocytes was found in the paracortical regions, HPL cells were located in lymphocytic follicles. In mycosis fungoidcs skin infiltrates (plaque stage), HPL+ lymphocytes predominated. Electron microscopy revealed homogeneously distributed HPL-receptor molecules on the cell surfaces of living lymphocytes in human lymph nodes. The HPL-receptor is thus proven to be related to the T lymphocyte subpopulation, and to provide a useful parameter for in situ lymphoid cell differentiation.
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