Insulin-like growth factor-I mRNA and peptide are distinctly confined to subtypes of macrophages, antigen-presenting cells, lymphocytes and HEV cells in non-neoplastic human lymph node

Abstract

Event Abstract Back to Event Insulin-like growth factor-I mRNA and peptide are distinctly confined to subtypes of macrophages, antigen-presenting cells, lymphocytes and HEV cells in non-neoplastic human lymph node Elisabeth Eppler1*, Dominique Oberlin1 and Christian Fellbaum2 1 University of Zürich, Institute of Anatomy, Switzerland 2 Hegau-Klinikum, Institute of Pathology, Germany IGF-I is a potent hormone that stimulates growth and differentiation and inhibits apoptosis in numerous tissues. Preliminary evidence suggests that IGF-I exerts differentiating, mitogenic and restoring activities also in the immune system. The present study investigates the cellular sites of IGF-I mRNA and peptide on archival human lymph node samples by routine staining, in situ hybridisation with an IGF-I probe, and immunohistochemistry with antisera specific for human IGF-I and CD3 (T-lymphocytes), CD20 (B-lymphocytes), CD68 (macrophages), CD21 (follicular dendritic cells, DC), S100 (interdigitating DC) and podoplanin (fibroblastic reticular cells). Numerous cells within the B- and T-cell compartments showed IGF¬I gene and peptide expression the majority of which was identified as macrophages. Solitary follicular DC exhibited IGF-I mRNA and peptide. B-lymphocytes did not contain IGF-I immunoreactive material, while few T-lymphocytes did. Furthermore, IGF-I mRNA and peptide-expressing cells were identified as high endothelial venule (HEV) cells. From this we conclude that the main task of IGF-I in human non-neoplastic lymph node may be autocrine and paracrine regulation of differentiation, stimulation and survival of lymphocytes, antigen-presenting cells and macrophages and differentiation and maintenance of HEV cells, and that the role for IGF-I in formation and sustaining of lymph node structures is more important than thought so far. Keywords: comparative endocrinology Conference: 25th Conference of the European Comparative Endocrinologists, Pécs, Hungary, 31 Aug - 4 Sep, 2010. Presentation Type: Conference Presentation Topic: Comparative endocrinology Citation: Eppler E, Oberlin D and Fellbaum C (2010). Insulin-like growth factor-I mRNA and peptide are distinctly confined to subtypes of macrophages, antigen-presenting cells, lymphocytes and HEV cells in non-neoplastic human lymph node. Front. Endocrinol. Conference Abstract: 25th Conference of the European Comparative Endocrinologists. doi: 10.3389/conf.fendo.2010.01.00008 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 26 Aug 2010; Published Online: 29 Aug 2010. * Correspondence: Dr. Elisabeth Eppler, University of Zürich, Institute of Anatomy, Zürich, Switzerland, elisabeth.eppler@ana.unibe.ch Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Elisabeth Eppler Dominique Oberlin Christian Fellbaum Google Elisabeth Eppler Dominique Oberlin Christian Fellbaum Google Scholar Elisabeth Eppler Dominique Oberlin Christian Fellbaum PubMed Elisabeth Eppler Dominique Oberlin Christian Fellbaum Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.