Abstract
Using statistical optimization, we enhanced the activity of phytase by a new Saccharomyces cerevisiae strain cultured in mineral medium. Concentrations of carbon source and inducer of phytase production were optimized using a 22 full factorial CCD and response surface methodology (RSM). Urea was fixed as nitrogen source in culture medium (0.15%, w/v). The culture medium consisting of 2.5% sucrose and 0.5% sodium phytate optimally supported the maximum phytase activity. In addition, we found that culture of the yeast at 35°C with shaking at 150 rpm supports maximum phytase production. The validity of this model was verified by culturing the organisms in flasks on a shaker. Using the optimized media and growth conditions, we obtained a 10-fold improvement in the production of phytase by S. cerevisiae.
Highlights
Phytate degradation is an important metabolic process in many biological systems
The yeast cultures were maintained in assay vials containing yeast malt agar (YMA) and stored at 4◦C until they were used for preparation of the preinoculum, which was done by suspending the spores in 2.5 mL of sterile water
Phytase production was measured using a colorimetric method by following the release of inorganic phosphate from phytic acid
Summary
Phytate is the major storage form of phosphorus found in cereals, grains, legumes, pollens, and seeds [1], it is not widely available to monogastric animals, such as swine and poultry. Phytic acid acts as an antinutrient because it chelates nutritionally important metals, such as iron, zinc, magnesium, and calcium and binds proteins and lipids, diminishing the bioavailability of these important nutrients [5]. Improving the digestibility of proteins and increasing the availability of phosphorus and other minerals, which are usually chelated by phytic acid [6], diminishes the antinutritive properties of phytate, and prevents environmental pollution. The ideal enzyme for the catalysis of such processes should have high catalytic specificity, resistance to proteolysis [7], and stability at elevated temperatures and under acidic conditions [8]
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