Abstract
Inclusion of mycological peptone in the base medium of X-gal plates facilitates the detection of isolates of Saccharomyces cerevisiae producing β-galactosidase due to improved colour development. Substitution of tryptone with mycological peptone in a standard Escherichia coli medium (LB) also facilitates colour development on X-gal plates and obviates the need for an inducer. The addition of mycological peptone to the base medium of X-gal plates offers a cost-effective method to improve the detection of the β-galactosidase gene in recombinant strains of S. cerevisiae and E.coli.
Published Version
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