Abstract

目的 比较可溶性血管内皮生成因子受体1(sFlt-1)胞外第2~3区和第2~4区对缺氧、高糖条件下血管内皮细胞增生、迁移及细胞外调节蛋白激酶1/2(ERK 1/2)信号转导通路的影响.方法 以逆转录聚合酶链反应(RT-PCR)法从人胎盘组织cDNA文库中扩增出sFlt-1胞外第2~3区和第2~4区,连接入pcDNA3.1载体后电泳,并进行测序鉴定;以羧甲基化葡聚糖(CMD)纳米磁颗粒为基因载体分别携带2种重组质粒,转染人脐静脉内皮细胞(UVECs)后分别进行正常、缺氧和高糖条件下的培养.MTT法检测各组人UVECs的增生;光学显微镜下观察人UVECs的迁移;Western blot法检测各组人UVECs ERK 1/2信号通路下游特异性磷酸化激酶ERK 1/2(p-ERK 1/2)蛋白的表达.结果 经过pcDNA3.1/sFlt-1(2-3)或pcDNA3.1/sFlt-1(2-4)转染的人UVECs在缺氧和高糖条件下的增生、迁移及p-ERK 1/2蛋白的表达较转染前均明显下降(P<0.01);2种重组质粒对缺氧和高糖条件下人UVECs增生、迁移及ERK 1/2信号转导通路的影响差异均无统计学意义(P>0.05).结论 sFlt-1受体胞外第2~3区和第2~4区均可抑制缺氧和高糖条件下人UVECs的增生、迁移及ERK 1/2信号通路的转导,且2种受体基因片段的作用无明显差异。

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