Impact of (Sodium) Aminophthalhydrazide on Insulin+ and Pdx1+ Rat Liver Cells in Experimental Model of Diabetes Mellitus Type I

Abstract

Aim: to evaluate quantity and functional activity of insulin+ hepatic cells in response to modulation of macrophage functional activity in rats in the modelling of diabetes mellitus type 1. Materials and methods: experiment was carried out on male Wistar rats, which were divided into 3 groups: intact, DM and DM+APH. To model DM alloxan in the dose of 170 mg/kg was injected intraperitoneally, the dose of APH was 2 mg/kg intramuscularly (a total of 20 injections within 30 days). Biochemical, enzyme immunoassay, immunohistochemical and statistical research methods were used. Results: сompare to the DM, in DM+APH group the increasing numbers of insulin+ hepatocytes and insulin+ sinusoidal cells were detected. At the same time, the total number of sinusoidal cells and Pdx1+ liver cells increase in both DM and DM+APH groups. It was found, that the content of insulin in the cytoplasm of insulin+ hepatocytes significantly higher in the DM+APH group, compare to intact and diabetic animals, which indicates the impact of APH at the growth of functional activity of insulin+ hepatocytes. An increase in the expression of insulin in the hepatic cells at the modulation of macrophage activity by APH may contribute to the correction of hyperglycemia in diabetic rats. Conclusion: introduction of APH leads to an increase in the number of insulin+ hepatocytes and their insulin-producing function, as well as insulin+ sinusoidal cells in animals with experimental diabetes. An increase in the total number of sinusoidal hepatic cells when the APH was administered in animals with alloxan-induces DM was revealed. At the same time, the action of APH enhances the expression of Pdx1 — a factor of differentiation for insulin-producing cells in the liver of diabetic animals.