Abstract
Organometallic complexes can decrease adhesion, migration, invasion of cancer cells, mainly through regulation of the extracellular matrix and therefore act against metastases. The aim was to investigate the anti-invasive properties of a rhenium-based metal compound, rhenium(I)-diselenoether (Re-diSe) and its effects on matrix metalloproteinase MMP-2, a key player in metastatic processes, in cultured MDA-MB231 triple-negative breast cancer cells. Matrigel was utilized to assess cancer cell adhesion to the extracellular matrix. Migration capacity was evaluated through the wound healing assay, while invasion assays were conducted using transwell invasion assay by the crystal violet dye. MMP-2 levels were quantified in the cell medium using a fluorometric kit assay. Cells were exposed to increasing concentrations of the drug (5, 10, 20, 50 and 100 μM). Three time points of drug exposure on the cells were investigated (48, 96 and 120 h). Re-diSe exhibited effectiveness even at doses as low as 5 or 10 μM in all biological assays. The time of exposure of 120 h significantly improved the effect on the invasion capacity of the cancer cells by comparison with 48 and 96 h. The results highlight the anti-invasive properties of Re-diSe and its impact on MMP-2 levels in MDA-MB231 breast cancer cells.
Published Version
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