Abstract
Morphological and biochemical responses to Acute Ozone Exposure (AOE) by Vigna unguiculata (L.) Walp. cultivar variety CO6 was studied. Plants were grown in controlled conditions and exposed to ozone at 40, 50, 60, 70, and 80 ppbv (T1-T5) for 15 min twice a day. Variation among shoot and root length, shoot-root ratio, fresh (FW), dry (DW) weight, leaf area (LA), leaf area ratio (LAR), number of guard cells (G), epidermal cells (E) (abaxial and adaxial), stomatal index (SI), chlorophyll a and b, total chlorophyll (TC), chlorophyll a/b ratio, chlorophyll protein ratio (CPR), ascorbic acid (AA), total phenol (TP), Proline (Pro), nitrate reductase activity (NRA) and Urease activity (UA) were measured in control and ozone-treated plants. The ozone treatment resulted in increase in dry weight, shoot length, leaf area, and number of epidermal cells, stomata and total chlorophyll on exposure up to 60 ppbv. Nitrate Reductase (EC: 1.6.6.3) and Urease (EC: 3.5.15) activities were inhibited by ozone.
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