Nitrate reductase, arginine deaminase, urease and dehydrogenase activities in natural soil (ridges with forest) and in cotton soil after acetamiprid treatments

Abstract

Soil enzymes are indicators of microbial activities in soil and are often considered as an indicator of soil health and fertility. They are very sensitive to the agricultural practices, pH of the soil, nutrients, inhibitors and weather conditions. To understand the effect of an insecticide, acetamiprid (IUPAC Name: (E)- N1-[(6-chloro-3-pyridyl) methyl]- N2-cyano- N1-methyl acetamidine) on different soil enzyme activities, the experiments were conducted for three consecutive years (2003–2005) at control and cotton experimental fields of Indian Agricultural Research Institute (IARI) and natural area (ridges with forest) in Delhi. The combined results for all three years were presented here to understand the impact of acetamiprid on soil enzyme activities. Acetamiprid was applied three times in one crop season after 41, 48 and 73 days of sowing, to control the pest. Soil of treated fields was analyzed for insecticide residues immediately after first insecticide treatment and thereafter at definite period. The residues of acetamiprid in experimental soil was varied from 0.30 ± 0.13 to 22.67 ± 0.2 μg g −1 d.wt. soil, during the crop period of 2003. The insecticide residues for 2004 ranged between 0.59 ± 0.38 and 13.42 ± 0.71 μg g −1 d.wt. soil and for 2005 it ranged between 0.48 ± 0.22 and 19.81 ± 0.33 μg g −1 d.wt. soil. An average half life of acetamiprid in our treated field was 11.2 ± 1.7 days for all three years. Similarly, the soil from natural area and control were also tested for insecticide residues. No detectable insecticide residues had been found. Soil from three localities i.e. natural, control and experimental fields were tested for different enzyme activities. Nitrate reductase, arginine deaminase, urease and dehydrogenase activities were high in natural soil in comparison to control soil and insecticide treated soil in all three experimental years. At the same time, nitrate reductase activity was all time low in acetamiprid treated soil. Acetamiprid had inhibitory effects on nitrate reductase, arginine deaminase and urease activities. After first treatment (43 days after crop sowing), nitrate reductase (41%), arginine deaminase (22%) and urease (35%) activities were declined. Dehydrogenase activity increased to 22% after first insecticide application. Enzyme activities were recovered at the end of each crop season. Therefore, it can be attributed that agricultural practices, weather conditions and use of acetamiprid might be responsible for the different level of enzyme activities in soil.