Abstract
Production of bioactive peptides from slaughterhouse wastes with a final low salt content by electrodialysis with bipolar membranes (EDBM) has recently been demonstrated as an innovative and efficient technology. However, a limitation in the electro-acidification process was noticed due to membrane fouling. In this context, the aim of the present work was to identify process conditions that could reduce peptide fouling in EDBM. Hence, two different conductivity levels (0.9 mS/cm and 4.5 mS/cm) were investigated in bipolar/monopolar (anionic and cationic) configuration and compared to conventional hydrolysis in the same conductivity conditions as well as a conventional hydrolysis using a buffer solution. The impact of such conductivity levels was studied during EDBM especially on membrane fouling and hydrolysis parameters: 1) kinetics of the enzymatic mechanism, 2) degree of hydrolysis and 3) peptide population. The results showed the same enzymatic mechanism “one-by-one” for all the conditions but a slower enzymatic kinetics for EDBM-0.9 mS/cm. EDBM-0.9 mS/cm allowed the production of bioactive peptides but with fouling formation on MCP while EDBM-4.5 mS/cm allowed the production of bioactive peptides without any fouling. Finally, optimizing certain conditions for further studies would lead to a promising green process to substitute synthetic additives used to protect food by antimicrobial peptides from blood slaughterhouses.
Published Version
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