Immunosensing platform with large detection range using an excessively tilted fiber grating coated with graphene oxide.

Abstract

We report an immunosensing platform with a large detection range using an excessively tilted fiber grating (ExTFG) coated with graphene oxide (GO). ExTFG was inscribed in standard single-mode fiber; GO film was coated on the fiber surface through hydrogen bond. The effectiveness and uniformity of GO deposited on the ExTFG surface were investigated by field emission scanning electron microscopy and energy spectrum method. Bovine serum albumin (BSA) monoclonal antibodies (MAbs) were used as biometric units to link the GO film through a covalent bond for the specific detection of BSA, so as to evaluate the performances of the proposed biosensor. The whole dynamic immobilization process of BSA MAbs and BSA detection were observed by the spectral evolution of the sensor. Experimental results show that the fabricated GO-coated ExTFG biosensor has a large detection range from 1.5nM-75nM and fast response for BSA antigen; the limit of detection is ∼0.88 nM by using an optical spectrum analyzer with a resolution of 0.03nm, and the dissociation constant KD and the affinity constant KA are calculated to be ∼6.66×10-9 M and ∼1.5×108 M-1, respectively. The proposed GO-coated ExTFG immunosensing platform could lay a foundation for the specific detection of other biomolecules.