Immunolocalization of TSOL18 and TSOL45-1A, the successful protective peptides against porcine cysticercosis, in Taenia solium oncospheres

Joel Martinez-Ocaña,Rina G De Kaminsky,Ana Flisser,Mirza Romero-Valdovinos,Pablo Maravilla

doi:10.1186/1756-3305-4-3

Abstract

Taenia solium life cycle includes humans as definitive hosts and pigs as intermediate hosts. One of the measures to stop the life cycle of this parasite is by vaccination of pigs. In experiments performed in pigs with TSOL18 and TSOL45-1A, two recombinant T. solium proteins, 99.5% and 97.0% protection was induced, respectively. The purpose of this paper was to localize these antigens in all stages of the parasite (adult worms, oncospheres and cysticerci) by immunofluorescence, with the use of antibodies against TSOL18 and TSOL45-1A that were obtained from the pigs used in the vaccination experiment. Results show that TSOL18 and TSOL45-1A are expressed on the surface of T. solium oncospheres and not in tapeworms or cysticerci, indicating that they are stage-specific antigens. This, therefore, might explain the high level of protection these antigens induce against pig cysticercosis.

Highlights

Taenia solium life cycle includes humans as definitive hosts and pigs as intermediate hosts
In order to localize the antigens on the parasite, blood samples from the pigs that were vaccinated with TSOL18, TSOL45-1A or Glutathione s- transferase (GST), were obtained prior and after immunization [3]
TSOL18 immunoglobulin G (IgG), TSOL45-1A IgG, GST IgG or pre-immune IgG, diluted 1:100 in the same blocking solution was added to the slide until all the section was covered and incubated overnight at 4oC

Summary

Introduction

Taenia solium life cycle includes humans as definitive hosts and pigs as intermediate hosts. * Correspondence: flisser@servidor.unam.mx 3Departamento de Microbiologia y Parasitologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico 04510 DF, Mexico Full list of author information is available at the end of the article education and lack of sanitation; it is prevalent in pork eating countries of Latin America, Asia and Africa, generating neurocysticercosis, the most frequent and devastating parasitic disease of the brain [2].

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Results

Conclusion