Abstract
Immobilization of macro molecules (such as enzymes) and micro-organisms can be generally defined as a procedure leading to their restricted mobility. Advantages of immobilization include easy separation of the enzymes/cells from the product and reuse of the enzymes/cells. In this research coconut tree leaf sheath was used to immobilize selected microbes which were used in fermentation tecnology. Coconut tree leaf sheath contains cellulose fiber layers which have cross linking between them. Sacchromyces cerevisiae was used as the microbial type due to widespread use in fermentation process. Microbes were entrapped within cellulose layers. Coconut tree leaf sheath was found to be an effecient solid support for immobilization. Immobilized microbes can be reused for fresh fermentation media. Immobilization can be carried out utilizing naturally avavilable coconut tree leaf sheath as a solid support, it`s usage is very cost-effective and eco-froiendly method rather than using synthetic or semi synthetic solid supports.
Highlights
Immobilization technique is a versatile and economical method that is used in industries [1]
Physical adsorption can be accomplished in two ways: non- specific adsorption and specific adsorption
Immobilization is carried out using synthetic resins or semi synthetic resins [3]
Summary
Immobilization technique is a versatile and economical method that is used in industries [1]. Non-specific adsorption is the simplest and easiest way of immobilization. Large numbers of pores are included among cellulose layers In these pores, cells of microorganism can be entrapped or can form non-specific bonds such as vander-waal bonds and hydrogen bonds with cellulose layers. On the other hand coconut leaf sheath is an ecofriendly, cost effective substance It is inert which do not show any adverse or toxic effect on micro-organisms. Coconut tree leaf sheath is 9000 tons per year in worldwide [7] It is sufficiently available in world wide. Coconut tree leaf sheath consist of cellulose layers (Figure 2) [8]. Due to –OH functional groups of cellulose, it can be induced inter and intra hydrogen bonding in between cellulose layers
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