Abstract
BackgroundIntermediates from processing sugar beets are considered an attractive feedstock for ethanol fermentation due to their high fermentable sugar content. In particular, medium prepared from raw sugar beet juice seems to be suitable for use in fermentation processes, but it is microbiologically unstable and requires sterilization.ResultsThis study investigates the effect of ozone treatment on the activity of microbial cells from Bacillus subtilis,Leuconostoc mesenteroides, Geobacillus stearothermophilus, Candida vini, and Aspergillus brasiliensis in raw sugar beet juice. Raw sugar beet juice contaminated with 105 cfu/mL of the microbial strains was treated with gaseous ozone (ozone concentration in the oxygen stream 0.1 g O3/L O2, flow rate 6 L/h, 10–30 min, 18–20 °C). The number of microflora decreased to 0 cfu/mL after 30 min of ozone treatment in all studied samples.ConclusionsMedium prepared from raw sugar beet juice and sterilized by ozonation is suitable for use in fermentation processes.
Highlights
Intermediates from processing sugar beets are considered an attractive feedstock for ethanol fermen‐ tation due to their high fermentable sugar content
Microorganisms and inoculations To evaluate the efficiency of the ozone sterilization process, the raw sugar beet juice was contaminated with the microbial strains Bacillus subtilis B01644, Leuconostoc mesenteroides ŁOCK 0964, Geobacillus stearothermophilus LOCK 0815 Candida vini syn
The parameters of the raw sugar beet juice obtained from Dobrzelin Sugar Factory were consistent with the literature [25]
Summary
This study investigates the effect of ozone treatment on the activity of microbial cells from Bacillus subtilis, Leuconostoc mesenteroides, Geobacillus stearothermophilus, Candida vini, and Aspergillus brasiliensis in raw sugar beet juice. Raw sugar beet juice contaminated with 105 cfu/mL of the microbial strains was treated with gaseous ozone (ozone concentration in the oxygen stream 0.1 g O3/L O2, flow rate 6 L/h, 10–30 min, 18–20 °C). The number of micro‐ flora decreased to 0 cfu/mL after 30 min of ozone treatment in all studied samples
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