Immobilization microbial cellulase and lipase as bio-catalyst agent using maltodextrin for biofuel synthesis

Abstract

The alternative method of using enzyme technology and modification methods will reduce the cost of biofuel production. New solution for biofuel production by improving the advantages from potential microbial to produce biocatalyst such as cellulase and lipase as an alternative catalyst for replacing the chemical catalyst for transesterification process. This study was developing new method of enzyme immobilization for feasibility biofuel production which is more efficient and effective process in industries. The result obtained in this work allowed concluding that the highest value for the total cellulolytic activity was obtain using carboxymethyl cellulose as substrate reaching value 4.225 U/ml for 24 h with optimization of pH 7 and temperature 50 °C. Instead of cellulase, the potential Aspergillus niger InaCC F538 can produce extracellular lipase and the value found for lipase activity in olive oil substrate is 3.710 U/ml for 24 h incubation time with optimization of pH 8 and temperature 50 °C. The immobilization process of cellulase and lipase using maltodextrin was enhancing the activity to 19.615 U/ml and 24.123 U/ml. The biofuel production based on microbial will be widely used for continuously used for renewable raw material from waste agriculture biomasses for biofuel production. New enzymes combination for improving enzyme stability for industrial application based on fermentation through the economically feasible.