IMMOBILIZATION AND STUDY OF TRYPSIN ON MAGNETITE-Fe3O4 NANOPARTICLE-BASED NATURAL AND SYNTHETIC POLYMER HYDROGELS

Abstract

The presented article designs hydrogel structures with magnetite nanoparticles in the composition, involving various synthetic and natural polymers. The synthesis of magnetite nanoparticles in the hydrogel composition was accomplished through the co-precipitation method, and the immobilization of protein using trypsin as a model drug was investigated. The incorporated of micro-quantities of magnetite Fe3O4 nanoparticles into the gel matrix enhances the affinity for the enzyme, resulting in a significant improvement in specific activity and stability compared to gels without magnetite Fe3O4 nanoparticles. In other words, increasing the degree of immobilization of magnetite Fe3O4 nanoparticles enhances both the immobilization degree and the biological activity of the enzyme. This phenomenon manifests as a continuous interaction between the gel composition and the magnetite nanoparticles of the enzyme