Enhanced fermentative production of Cephalosporin C by magnetite nanoparticles in culture ofAcremonium chrysogenum

Abstract

Magnetite (Fe3O4) nanoparticles (MNPs) have many applications in bioprocesses. This study investigated bioprocess production of Cephalosporin C (CPC) by Acremonium chrysogenum. The effect of MNPs was tested for enhancing the fermentation process. MNPs were synthetised by a straightforward method of co-precipitation. Various concentrations of MNP (0, 0.0025, 0.005, 0.01, 0.02 and 0.04 g/l) were added to fermentation media of the strain. During fermentation, evaluations were taken for titer of CPC, biomass, pH value and morphology of the strain. Comparison was made between CPC produced in the control medium and that in the nanoparticle enriched media from several consecutive batches; it was determined that MNP addition had a positive effect on the bioprocess and enhanced titer production. The titer of CPC in MNP-containing medium (0.04 g/l) increased by 60% compared with MNP-free medium. These results show that MNPs present good potential for improving bioprocesses and enhancing productivity of CPC fermentation by A. chrysogenum.