Abstract
Optimization of an environmental factor (pH, light intensity and agitation) for Hydrogen gas production using immobilized bacteria photosynthetic of Rhodobium marinum through facultative-photo-fermentation was studied. The experiment was conducted by using CRD (completely random design) to understand the effect of each selected parameter and their combination. The result shows that the combination of light intensity, pH and agitation were significantly affected the hydrogen production. Further data analyses gave information that light intensity and pH treatments were regulating the substrate consumption, i.e. accelerating the glucose digestion, which was, enhance the hydrogen gas production. The highest production of hydrogen gas from the experiment was in the range of 1055 - 1200 mL/L, at glucose, contain 10 g/L in the substrate. The optimizations of the photo-fermentation adaptive processes for bioproduction hydrogen gas are a necessary step to find the optimum condition of hydrogen rate and yield, in a certain period. Hydrogen gas is one of the powerful energy carriers that easy to be converting into energy form dailies such as electricity, fuel, heat or storage battery.
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