Abstract
Microfluidic/optofluidic microscopy is a versatile modality for imaging and analyzing properties of cells/particles while they are in flow. In this paper, we demonstrate the integration of fused silica microfluidics fabricated using femtosecond laser machining into optofluidic imaging systems. By using glass for the sample stage of our microscope, we have exploited its superior optical quality for imaging and bio-compatibility. By integrating these glass microfluidic devices into a custom-built bright field microscope, we have been able to image red blood cells in flow with high-throughputs and good fidelity. In addition, we also demonstrate imaging as well as detection of fluorescent beads with these microfluidic devices.
Highlights
I MAGING Flow Cytometry (IFC) is a versatile platform that combines the benefits of digital microscopy and flow cytometry
We report our investigations on the usability of femtosecond laser micromachined glass microfluidic devices in optofluidic imaging systems
We have demonstrated the applicability of femtosecond laser micromachined microfluidic devices for IFC systems
Summary
I MAGING Flow Cytometry (IFC) is a versatile platform that combines the benefits of digital microscopy and flow cytometry. In most of the previously demonstrated IFC systems [4], [9]–[11], microfluidic devices fabricated in Polydimethylsiloxane (PDMS) have been used for sample handling. The advent of femtosecond laser micromachining/ultra-fast laser inscription (ULI) has enabled rapid prototyping of three dimensional glass microfluidic devices [20]. We report our investigations on the usability of femtosecond laser micromachined glass microfluidic devices in optofluidic imaging systems. JAGANNADH et al.: IMAGING FLOW CYTOMETRY WITH FEMTOSECOND LASER-MICROMACHINED GLASS MICROFLUIDIC CHANNELS. We outline the process used to fabricate the microfluidic devices and demonstrate the usability of these devices in bright-field and fluorescence microfluidic microscopy imaging modalities
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