Imaging Bacillus anthracis Edema Toxin Effects on Normalized Murine Dendritic Cells and Macrophages

Abstract

Event Abstract Back to Event Imaging Bacillus anthracis Edema Toxin Effects on Normalized Murine Dendritic Cells and Macrophages Yannick Trescos1, 2*, Danel Fiole1, Marion Mulier1 and Jean-Nicolas Tournier1, 2* 1 Institut de Recherche Biomédicale des Armées (IRBA), Department of microbiology, France 2 Ecole du Val-de-Grâce (EVDG), France Virulence of Bacillus anthracis, the agent of anthrax is caused by two main factors, the capsule and two toxins. One of these toxins, edema toxin (ET) is an adenylate cyclase, producing a rise in intracellular cAMP concentrations. ET deregulates the immune system but little is known on its effects on immune cell cytoskeleton. Using adhesive micropattern array, initially designed for cells capable of stretching on micropatterns and forming stress fibers, we have developed a method of depositing immune cells to normalize their morphology. First, we present an innovative, robust and sensitive model to study murine bone-marrow-dendritic cells (BMDCs) and macrophages (BMDMs) in vitro, based on their adhesion to micro-patterns to standardize their morphology. This model reduces the variability of shape inherent in our cell cultures and gain sensitivity and reproducibility. Thus, the quantification of sub-cellular phenomena has become possible for studying many functions of immune cells. Second, our micro-patterning model has been used to identify and quantify the effects of ET on cytoskeleton of BMDCs and BMDMs. We observed that ET caused a time-dependent retraction of the actin cytoskeleton on both cells. This retraction begins with the formation of actin-rich cortical protrusions, appearing like membranes ruffles. This reorganization is accompanied by a perinuclear accumulation of actin, responsible of a deformation of the nucleus. We thus infer that our imaging model on immune cells opens new perspectives for high throughput analysis of morphology and functions of BMDCs and BMDMs in vitro. Acknowledgements This presentation was made possible by Direction Générale de l’Armement funding and ANR ANABIO. Keywords: Anthrax, Dendritic Cells, Macrophages, micropatterning, edema toxin, Cytoskeleton Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Trescos Y, Fiole D, Mulier M and Tournier J (2013). Imaging Bacillus anthracis Edema Toxin Effects on Normalized Murine Dendritic Cells and Macrophages. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01089 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 29 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Yannick Trescos, Institut de Recherche Biomédicale des Armées (IRBA), Department of microbiology, Brétigny-sur-Orge, 91220, France, yannick.trescos@yahoo.fr Prof. Jean-Nicolas Tournier, Institut de Recherche Biomédicale des Armées (IRBA), Department of microbiology, Brétigny-sur-Orge, 91220, France, jntournier@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Yannick Trescos Danel Fiole Marion Mulier Jean-Nicolas Tournier Google Yannick Trescos Danel Fiole Marion Mulier Jean-Nicolas Tournier Google Scholar Yannick Trescos Danel Fiole Marion Mulier Jean-Nicolas Tournier PubMed Yannick Trescos Danel Fiole Marion Mulier Jean-Nicolas Tournier Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.