Mechanistic insights into H. pylori pathogenesis and host response

Abstract

Event Abstract Back to Event Mechanistic insights into H. pylori pathogenesis and host response Bow Ho1* 1 Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore Helicobacter pylori is a Gram negative bacterial gastric pathogen that infects ~50% of the world’s population with some progressing to gastric cancer. The indelible link between Helicobacter pylori and severe gastric diseases coupled with the emergence of multiple-antibiotic resistant H. pylori and the high prevalence of gastric cancer necessitate urgent attention. Furthermore, the mechanistic action of pathogenesis has not been firmly established. Among the known pathogenic factors of H. pylori reported, cytotoxin associated gene A (CagA), an oncoprotein, has been cited to induce inflammatory response in host cells and cause disruption of gastric epithelial cell-cell tight junctions. We report here a surface localized heat shock protein that effects the cytoskeleton rearrangement of cells independently, and exacerbates disruption of cell-cell tight junctions through its interaction with CagA. This protein is found in all strains of H. pylori analysed, and that it aids the bacteria in colonizing BALB/c mice. The dynamics of interaction between heat shock protein and CagA intrabacterially, as well as between the bacterial pathogen and the host in the course of H. pylori pathogenesis will be discussed. Acknowledgements This work was supported by Grant No. R182000180213. References Amieva MR, Vogelmann R, Covacci A, Tompkins LS, Nelson WJ, et al. (2003) Disruption of the epithelial apical-junctional complex by Helicobacter pylori CagA. Science 300: 1430-1434. Covacci A, Censini S, Bugnoli M, Petracca R, Burroni D, et al. (1993) Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc Natl Acad Sci U S A 90: 5791-5795. Du RJ, Ho B (2003) Surface localized Heat Shock Protein 20 (HslV) of Helicobacter pylori. Helicobacter 8: 257-267. Gong M, Ling SS, Lui SY, Yeoh KG, Ho B (2010) Helicobacter pylori gamma-glutamyl transpeptidase is a pathogenic factor in the development of peptic ulcer disease. Gastroenterology 139: 564-573. Hatakeyama M (2008) Saga of CagA in Helicobacter pylori pathogenesis. Curr Opin Microbiol 11: 30-37. Kwok T, Zabler D, Urman S, Rohde M, Hartig R, et al. (2007) Helicobacter exploits integrin for type IV secretion and kinase activation. Nature 449: 862-866. Murata-Kamiya N, Kurashima Y, Teishikata Y, Yamahashi Y, Saito Y, et al. (2007) Helicobacter pylori CagA interacts with E-cadherin and deregulates the beta-catenin signal that promotes intestinal transdifferentiation in gastric epithelial cells. Oncogene 26: 4617-4626. Keywords: Helicobacter pylori, CagA, Cell-cell junction disruption, Heat Shock Protein 20, Pathogenesis Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Ho B (2013). Mechanistic insights into H. pylori pathogenesis and host response. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00478 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 02 Apr 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Bow Ho, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore, michob@nus.edu.sg Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Bow Ho Google Bow Ho Google Scholar Bow Ho PubMed Bow Ho Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.