An adjuvant, fungal immunomodulatory protein (FIP)-fve, potentiates the immune response by engaging the co-stimulatory molecules, CD27 and CD134 (OX-40)

Abstract

Event Abstract Back to Event An adjuvant, fungal immunomodulatory protein (FIP)-fve, potentiates the immune response by engaging the co-stimulatory molecules, CD27 and CD134 (OX-40) Haris Ong1, Kuo I-Chun1* and Huang Chiung-Hui1* 1 National University of Singapore, Paediatrics, Singapore Fungal immunomodulatory protein (FIP)-fve has been shown to be an immunomodulator in tumor immunology and allergy. However, the molecular mechanism by which FIP-fve modulates the immune response to exert its adjuvant effect is yet to be elucidated. Our in vitro results demonstrated that FIP-fve required the interaction between mouse T cells and bone marrow-derived dendritic cells (BM-DCs) to induce the optimal T cell proliferation and secretion of several pro-inflammatory cytokines and chemokines, such as IFN-γ, MIG, MIP-1α, MIP-1β, and IP-10. In addition, we found that FIP-fve could directly activate T cells in a dose- and time- dependent manner, shown by the up-regulation of CD69, CD25, CD134 (OX-40), CD137 (4-1BB) on both gated CD4+T and CD8+T cells as well as CD27, CD28 on gated CD8+T cells mostly. Interestingly, blocking of the interaction between CD134 (OX-40), CD27 and their corresponding ligands significantly reduced the FIP-fve-induced T cell proliferation and cytokine/chemokine secretion. Furthermore, when FIP-fve was injected into the mouse footpad, we observed that the absolute cell number of CD4+T cells, CD8+T cells, and B cells were increased tremendously in the draining lymph node. However, our in vivo bromodeoxyuridine (BrdU)-incorporation assay suggested that the proliferating cells upon FIP-fve injection were mostly CD8+T cells and CD4+ T cells. To confirm the T cell activation in draining lymph node, FIP-fve was also shown to up-regulate the expression of CD69 and the co-stimulatory molecules, CD27 and CD28, on the gated T cells. Thus, FIP-fve may function as an adjuvant via activation and modulation of immune system. Keywords: adjuvants, FIP-fve, CD8+ T cell, CD4+ T cells, CD27, CD134 (OX-40), Fungal immunomodulatory protein Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Ong H, I-Chun K and Chiung-Hui H (2013). An adjuvant, fungal immunomodulatory protein (FIP)-fve, potentiates the immune response by engaging the co-stimulatory molecules, CD27 and CD134 (OX-40). Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00110 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Kuo I-Chun, National University of Singapore, Paediatrics, Singapore, 117597, Singapore, paekic@nus.edu.sg Dr. Huang Chiung-Hui, National University of Singapore, Paediatrics, Singapore, 117597, Singapore, paehch@nus.edu.sg Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Haris Ong Kuo I-Chun Huang Chiung-Hui Google Haris Ong Kuo I-Chun Huang Chiung-Hui Google Scholar Haris Ong Kuo I-Chun Huang Chiung-Hui PubMed Haris Ong Kuo I-Chun Huang Chiung-Hui Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.