Proteus mirabilis flagellin, neither a good adjuvant nor a protective antigen against urinary tract infection

Abstract

Event Abstract Back to Event Proteus mirabilis flagellin, neither a good adjuvant nor a protective antigen against urinary tract infection Paola Scavone1*, Ana Umpiérrez1, Analía Rial2, José A. Chabalgoity2 and Pablo Zunino1 1 Instituto de Investigaciones Biológicas Clemente Estable, Department of Microbiology, Uruguay 2 Instituto de Higiene, Department of Biotecnology, Uruguay P. mirabilis, a gram negative bacterium associated with complicated urinary tract infections (UTI), expresses several virulence factors including MR/P fimbriae and flagella. Flagellin from certain bacteria has shown interesting adjuvant and protective properties but P. mirabilis flagellin has not been analyzed so far. Here, we evaluated the role of P. mirabilis flagellin as protective antigen in a mouse UTI model, and its adjuvant capacity for the protective antigen MrpA (MR/P fimbriae structural subunit). Four groups of mice were intranasally immunised at days 0, 3, 10, 17 and 24 with either MrpA, flagellin, both proteins and PBS only. Before and after immunisation urine and blood samples were collected to evaluate antibodies induction. Cytokine production was analysed on day 30 in splenocytes. At day 31 mice were challenged using an ascending P. mirabilis UTI model, being sacrificed 7 days after for bacteria quantification in kidneys and bladders. MrpA-immunised mice showed a significant increase in serum and urine specific IgG and IgA while mice immunised with MrpA-flagellin or flagellin did not. Animals did not show significant anti-flagellin antibodies induction. MrpA-immunised showed a significant decrease in bacteria recovered from bladders and kidneys compared with any other group of mice. Instead, infection levels in flagellin, MrpA-flagellin and PBS-immunised mice showed no significant differences between them. Remarkably, IL-10 was significantly induced in the mice receiving flagellin or MrpA-flagellin. These results showed that P. mirabilis flagellin is not a good adjuvant and did not protect mice against UTI, and suggested that it may have an immunomodulatory role. Keywords: Flagellin, Urinary Tract Infections, adjuvants, Vaccines, MrpA antigen Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Scavone P, Umpiérrez A, Rial A, Chabalgoity JA and Zunino P (2013). Proteus mirabilis flagellin, neither a good adjuvant nor a protective antigen against urinary tract infection. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00445 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Ms. Paola Scavone, Instituto de Investigaciones Biológicas Clemente Estable, Department of Microbiology, Montevideo, Montevideo, 11600, Uruguay, pscavone@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Paola Scavone Ana Umpiérrez Analía Rial José A Chabalgoity Pablo Zunino Google Paola Scavone Ana Umpiérrez Analía Rial José A Chabalgoity Pablo Zunino Google Scholar Paola Scavone Ana Umpiérrez Analía Rial José A Chabalgoity Pablo Zunino PubMed Paola Scavone Ana Umpiérrez Analía Rial José A Chabalgoity Pablo Zunino Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.