Dynamics of the immune response to DosR and Rpf antigens of Mycobacterium tuberculosis in house hold contacts of tuberculosis patients

Abstract

Event Abstract Back to Event Dynamics of the immune response to DosR and Rpf antigens of Mycobacterium tuberculosis in house hold contacts of tuberculosis patients Leonar Arroyo1, Blanca L. Ortiz1, Mauricio Rojas1, 2, Luis F. Garcia1, Tom H. Ottenhoff3 and Luis F. Barrera1* 1 Universidad de Antioquia, Instituto de Investigaciones Médicas, Colombia 2 Universidad de Antioquia, Colombia 3 Leiden University Medical Center, Dept. of Infectious Diseases, Netherlands Introduction. Previously, we found a predominance of T CD4+ response with a TCM phenotype to selected DosR and Rpf antigens of M. tuberculosis (Mtb) in LTBI subjects (> 3 years), suggesting that this response may be important to maintain latent infection. To better understand the development of this response, we followed-up for one year the T CD4 and T CD8 expressing iIFNg, the memory phenotype, and cytokine production in HHC of recently diagnosed TB patients. Methods. PBMCs from HHC were cultured for 7 days in presence/absence of DosR (Rv1737c, Rv2029c and Rv2628), Rpf (Rv0867c and Rv2389c), and control ESAT6-CFP10 (E6-C10) and PPD antigens. The frequency of T CD4+IFNg+, T CD8+IFNg+, and memory phenotype (CD45RO and CD27) was established by flow cytometry. The cytokine production (IL-2, IL-4, IL-5, IL-10, IL-13, IL-15, IL-17, IFNg and MIP-1α) was determined by the Luminex technology. Main findings. A significant decrease in the frequency of T CD4+IFNg+ cells after 12 months in response to Rv1737c and Rv2029c, and E6-C10 was observed, concomitant with an increase in the frequency of CD4+IFNg+ T cells with a TCM phenotype. The levels of IFNg, IL-10, IL-17, and TNFα in response to both DosR and Rpf antigens decreased significantly after 12 months of the follow-up. Conclusions. The decreased frequency of CD4+IFNg+ T cells and cytokine production may be an indication of an attenuated inflammatory response to Mtb infection. The increased frequency of CD4+IFNg+ with a TCM phenotype could be crucial to the control of Mtb infection and TB reactivation. Funding: resources Colciencias, Grant 1115-4592-1461 Keywords: Tuberculosis, latency, immune response, dosr, Rpf Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Arroyo L, Ortiz BL, Rojas M, Garcia LF, Ottenhoff TH and Barrera LF (2013). Dynamics of the immune response to DosR and Rpf antigens of Mycobacterium tuberculosis in house hold contacts of tuberculosis patients. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00704 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Luis F Barrera, Universidad de Antioquia, Instituto de Investigaciones Médicas, Medellin, 05001000, Colombia, luis.barrera@udea.edu.co Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Leonar Arroyo Blanca L Ortiz Mauricio Rojas Luis F Garcia Tom H Ottenhoff Luis F Barrera Google Leonar Arroyo Blanca L Ortiz Mauricio Rojas Luis F Garcia Tom H Ottenhoff Luis F Barrera Google Scholar Leonar Arroyo Blanca L Ortiz Mauricio Rojas Luis F Garcia Tom H Ottenhoff Luis F Barrera PubMed Leonar Arroyo Blanca L Ortiz Mauricio Rojas Luis F Garcia Tom H Ottenhoff Luis F Barrera Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.