Identifikasi Galur dan Gen-gen Terkait Toleran Kekeringan pada Padi Transgenik cv. T309 yang Mengandung Vektor Penanda Aktivasi

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<p>Activation tagging is an efficient tool for<br />functional analysis of the rice genes. We have developed a<br />number of transgenic rice lines (Oryza sativa L. ssp.<br />japonica cv. Taipei 309) containing activation tagging vector.<br />However, the phenotypes and genotypes of these lines, in<br />relation to the drought stress, have not been analyzed. The<br />objectives of this research were to identify transgenic rice<br />lines that showed tolerance to the drought stress and to<br />identify the genes that may be associated with the drought<br />stress. The drought stress tolerance in transgenic rice lines<br />was identified by testing their tolerance to the drought stress<br />and also by detecting the presence of bar and nptII genes.<br />The result showed that 56 out of 59 rice lines were resistant<br />to Basta herbicide and three of them showed tolerance to<br />drought stress, namely PA.T-1.2, PA.T-4.1, and PA.T-5.1 lines.<br />PCR analysis showed that PA.T-1.2 and PA.T-4.1 contained<br />both hptII and bar genes, while the PA.T-5.1 line contained<br />bar gene only. Thermal Asymetric Interlaced-PCR (TAILPCR)<br />analysis showed that two genes may be asssociated<br />with the drought stress tolerance. Those genes are<br />OSJNBa0004120.14 that produces uridylate putative kinase<br />and OsPPCK2L that produces phosphoenolpyruvate<br />carboxylase kinase.</p>